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Site-directed Mutagenesis Service

Site-directed mutagenesis is essential to molecular biology in studies of gene regulatory elements, DNA-protein interaction, protein structure and its function, enzyme active sites and novel proteins. BioInnovatise's site-directed mutagenesis services include point mutations, deletions, and insertions. Our high quality work, quick delivery, low service prices and FREE  shipment for local institutes, universities and companies are unbeatable. We will match any lowest prices in biotech industry and efficiently deliver your mutant construct with minimum turnaround time.

Advantages

Sample Submission Requirements

Please provide following:

Delivery

Our delivery package includes:

Quotations and ordering

For quotation or any other inquiry, please contact us by email, phone or fax.

 

Random Mutagenesis

Random mutagenesis is a powerful tool to determine protein structure-function relationships and to improve or alter their characteristics by modifying proteins. Error-prone PCR is a random mutagenesis technique for generating amino acid substitutions in proteins by introducing mutations into a gene during PCR. Mutations are deliberately introduced by error- prone DNA polymerases. After the mutated PCR products are cloned into an expression vector, the resulting mutant library can be screened for changes in protein activity. Due to the lack of structural information, or when such mutations are difficult to predict from protein structure, the Scientists can use random mutagenesis to identify beneficial mutations. We offer to create a random mutagenesis library of any desired complexity to any gene of your interest.

Service Description:

Site-Directed Random Mutagenesis

Site-Directed Random Mutagenesis is also called Site-Saturation Mutagenesis or Targeted Random Mutagenesis or Domain Mutagenesis. Since conventional random mutagenesis generates large numbers of non-functional mutants rather than functional genes, these approaches inevitably require extensive screening, a highly cost and long time. By targeting specific residues or protein domain, most of codons remain as wild-type, which can make the mutants maintain natural structure of protein backbone despite of hyper diversity of functional region. Therefore, the mutant library can contain high portion of functional mutants that are most likely to yield positive results.

Service Description:

 

Email: Info@bioinnovatise.com
Phone: 1-301-230-2268
Fax: 1-301-230-2267
Website: www.BioInnovatise.com

Custom Cloning service

FACE-TO-FACE TALK with our experienced scientist.

FREE door-to-door delivery*.

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Site-Directed Mutagenesis Service

Mutations on large plasmids up to 18.0 kb.

FREE design and oligo synthesis.

FREE door-to-door delivery*.

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