Site-directed Mutagenesis Service

Site-directed mutagenesis is essential to molecular biology in studies of gene regulatory elements, DNA-protein interaction, protein structure and its function, enzyme active sites and novel proteins. BioInnovatise's site-directed mutagenesis services include point mutations, deletions, and insertions. Our high quality work, quick delivery, low service prices and FREE  shipment for local institutes, universities and companies are unbeatable. We will match any lowest prices in biotech industry and efficiently deliver your mutant construct with minimum turnaround time.

Advantages

• Mutation on Large Plasmid: Any point mutations, insertions and deletions can be made on up to 18 kb plasmid with our most advanced mutagenesis technology.
• Highest Accuracy: BioInnovatise's high-fidelity DNA polymerase ensures accuracy of mutants and guarantees NO any unwanted mutations.
• Multiple mutation sites can be introduced in one mutagenesis reaction.
• NO charges if we cannot deliver your mutant constructs.
• Most Competitive price: Low service prices are unbeatable. NO ANY HIDING FEES AND NO UPFRONT PAYMENTS.

Sample Submission Requirements

Please provide following:

• The complete sequence of the template (the target)
• Mutation specifications
• Maps and antibiotic resistance of the template and destination vectors
• ~3 ��g of wildtype plasmid DNA

Delivery

Our delivery package includes:

• 2 - 4 ug of high pure mutant construct;
• Project report with sequence alignments and original sequencing data (electronic).
• Our typical turnaround is about 7 business days.
• We offer FREE door-to-door delivery as well as sample collection for the customers in NIH Bethesda and Twinbrook campuses and other local Universities, Institutes and biotech companies.

Quotations and ordering

For quotation or any other inquiry, please contact us by email or phone

 

Random Mutagenesis

Random mutagenesis is a powerful tool to determine protein structure-function relationships and to improve or alter their characteristics by modifying proteins. Error-prone PCR is a random mutagenesis technique for generating amino acid substitutions in proteins by introducing mutations into a gene during PCR. Mutations are deliberately introduced by error- prone DNA polymerases. After the mutated PCR products are cloned into an expression vector, the resulting mutant library can be screened for changes in protein activity. Due to the lack of structural information, or when such mutations are difficult to predict from protein structure, the Scientists can use random mutagenesis to identify beneficial mutations. We offer to create a random mutagenesis library of any desired complexity to any gene of your interest.

Service Description:

• Cloning of the randomized DNA into expression vectors.
• Cloning into any other vectors.
• Confirmation of desired mutation by DNA sequencing.
• Selection of mutation frequencies depending on the application of the study.
• Titration of your randomly mutated plasmid library.
• Upon special request, we can provide library screening and protein activity assay, etc. with additional service fees.

Site-Directed Random Mutagenesis

Site-Directed Random Mutagenesis is also called Site-Saturation Mutagenesis or Targeted Random Mutagenesis or Domain Mutagenesis. Since conventional random mutagenesis generates large numbers of non-functional mutants rather than functional genes, these approaches inevitably require extensive screening, a highly cost and long time. By targeting specific residues or protein domain, most of codons remain as wild-type, which can make the mutants maintain natural structure of protein backbone despite of hyper diversity of functional region. Therefore, the mutant library can contain high portion of functional mutants that are most likely to yield positive results.

Service Description:

• Cloning of the site-directed randomized DNA into expression vectors.
• Cloning into any other vectors.
• Confirmation of site-directed random mutations by DNA sequencing.
• Selection of site-directed mutation frequencies depending on the application of the study.
• Titration of your site-directed randomly mutated plasmid library.
• Upon special request, we can provide library screening and protein activity assay, etc. with additional service fees.