Lentiviral Library
BioInnovatise Viral Vector Team
Updated May 13, 2024
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Lentiviral library refers to a collection of lentiviral vectors that collectively encode a diverse set of genetic elements including genes, shRNAs (short hairpin RNAs), or CRISPR-Cas9 guide RNAs. These libraries offer researchers the opportunity to systematically interrogating gene function and regulatory networks on a genome-wide scale. Researchers can perform large scale functional genomics studies with high throughput and scalability.
How Is A Lentiviral Library Constructed?
Lentiviral libraries are typically generated by cloning individual genetic elements (such as those listed above) into lentiviral vector backbones, which are then packaged into lentiviral particles for delivery into target cells. Each lentiviral vector within the library carries a different genetic element, allowing researchers to introduce a diverse range of genetic perturbations into a population of cells.
There are many different types of libraries available for researchers and are chosen based on their research application. Our team has assembled this reference table that summarizes the different types of lentiviral libraries and their research goals:
| Type Of Lentiviral Library | Library Components | Research Goals |
|---|---|---|
| cDNA Libraries | These libraries consist of lentiviral vectors encoding complementary DNA sequences corresponding to genes of interest. | Used to overexpress genes or gene variants in target cells for functional studies or screening experiments. |
| shRNA Libraries | These libraries consist of small RNA molecules that can silence gene expression by triggering the degradation of target mRNA. | Used for loss-of-function screens to identify genes involved in biological processes or disease phenotypes. |
| CRISPR-Cas9 Libraries | These libraries consist of lentivirus vectors encoding guide RNAs (gRNAs) that target specific genomic loci for editing by the CRISPR-Cas9 system. | Used for high throughput genome wide knock out or activation (knock in) screens to identify genes essential for cellular processes or disease states. |
| cDNA ORF Libraries | These libraries consist of lentiviral vectors encoding open reading frames (ORFs) of genes. | Used for allowing for the overexpression of full-length proteins or protein domains in target cells |
If you are interested in having a CRISPR-Cas9 knock-out or knock-in performed on your plasmid DNA construct, learn about our CRISPR-Cas9 services.
The above diagram illustrates the production process for lentivirus packaging BioInnovatise, where our team uses a HEK293T cell line for lentivirus packaging cells.
If you are interested in starting a lentiviral library construction production, please provide the following when requesting a production:
- 3 µg of wildtype plasmid DNA
- The library parameters and protocol needed for your research
If you are unsure which lentiviral library is right for your research application, contact our viral vector team.
Learn about our quick turnaround lentivirus packaging services.
Want to learn more about the latest in lentivirus based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on lentivirus-based research.
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