CRISPR-Cas9 Services

Quick Turnaround CRISPR Vectors, Cassette Design, and Gene Knock-Out and Knock-In Services for Research and Development

Plasmid DNA Constructs Cloned

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Viral Vectors Packaged

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Plasmid DNA Preparations

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Let's Start Your CRISPR-Cas9 Project

Unlock precise genome editing with our comprehensive CRISPR-Cas9 toolkit, featuring customizable vectors, expert sgRNA design, and specialized cassette production services. Whether you need to knock out genes or precisely insert new sequences, our experienced CRISPR team delivers complete solutions tailored to your research goals, backed by our versatile collection of validated expression vectors.

CRISPR-Cas9 service lead times can change, so be sure to check our current lead times.

Our CRISPR Vector Collection

CRISPR-Cas9 vectors are genetic constructs used in genome editing. Our CRISPR team has designed a series of these constructs to deliver the necessary components of the CRISPR system into cells for targeted gene editing. A typical CRISPR vector includes the following components: Cas9 gene, gRNA expression cassette, selectable marker genes, (optional) reporter genes, origin of replication, polyadenylation signal, and (optional) homology arms.

If you’re interested in producing your own custom CRISPR-Cas9 vector, our CRISPR team is ready to start your production. Our team has put together a list of frequently asked questions and answers about CRISPR vectors if you have questions.

Below is a list of the CRIPSR vectors we currently provide. If you’re unsure which vector is right for your research, our CRISPR team is happy to discuss your project with you.

Vector	Description
pGPS-Cas-T2A-Puro	In vivo expression sgRNA and Cas9
pGPS-Nickase-T2A-Puro	In vivo expression sgRNA and Nickase Cas9
pGPS-T7-sgRNA	T7 promoter in vitro sgRNA production
pGPS-T7-Cas9	T7 promoter in vitro Cas9 RNA production
pGPS-Cas	In vivo Cas9 expression

sgRNA Construct Cloning Services

Our cloning team is also able to assist in designing, generating, and cloning sgRNAs for CRISPR-Cas9 gene-editing experiments. You can choose any of the above sgRNA expression vectors or a custom vector free of charge.

Cassette Production and Donor Vector Construction Design Services

CRISPR cassettes are essential components in the CRISPR-Cas9 genome editing system. They are used to deliver the necessary genetic elements, including the Cas nuclease (e.g., Cas9) and guide RNA (gRNA), into the target cells or organisms to perform precise gene editing. Our team can design and construct the best donor vector in homology-directed repair. You can select a pre-designed cassette or request a custom cassette for the tag or drug selection. Below is a list of the standard donor cassettes we currently provide. Learn more about CRISPR-Cas9 cassettes.

Cassette	Vector
Cassette 1	CMV-GFP-T2A-Puro-PA
Cassette 2	CMV-mCherry-T2A-Blasticidin-PA
Cassette 3	Luciferase-CMV-GFP-T2A-Puro-PA

CRISPR Knock-Out and CRISPR Knock-In Services

CRISPR knock-out aims to disrupt the function of a gene, often by introducing mutations that render the gene non-functional, while CRISPR knock-in aims to insert a specific genetic sequence into the genome to achieve a desired genetic change or addition.

Our CRISPR knock-out or knock-in services are a whole product solution for researchers to knock-out or knock-in a gene of interest. Our production deliverables contain a plasmid that expresses a specific gRNA and Cas9 protein, a negative, and a donor vector with Luciferase-CMV-GFP-T2A-Puro-PA cassette (unless otherwise noted) that allow drug selection of positive clones. Learn why a CRISPR-Cas9 service is preferrable to a DIY kit.

Not sure which service is right for your research? The table below outlines the key differences.

CRISPR Knock-Out Service
Objective	Knock-out productions disrupt or deactivate (“knock out”) the function of a specific gene by introducing small insertions or deletions (indels) into a target gene’s DNA sequence. These indels can introduce frame-shift mutations that often result in non-functional proteins or lead to premature stop codons.
Outcome	The gene’s function is disrupted and it may no longer produce a functional protein, effectively “turning off” the gene.
Applications	Knock-out genome editing is used to study gene function, model diseases, and explore the consequences of gene inactivation. It is also used as a therapeutic strategy for diseases caused by overactive or harmful genes.

CRISPR Knock-In Service
Objective	Knock-in productions insert or add (“knock in”) a specific genetic sequence (usually a new or modified DNA segment) into a genome at a precise location, introducing a desired genetic change, such as adding a functional gene or correcting a mutated gene.
Outcome	A specific genetic sequence is integrated into the target gene or genomic location, leading to the expression of a modified or additional gene product.
Applications	Knock-in genome editing is used for various purposes, including introducing reporter genes, tagging proteins for tracking or visualization, correcting disease-causing mutations, or adding specific sequences for therapeutic purposes.

Sample Submission Requirements for Knock-Out or Knock-In Services

To get started on your CRISPR-Cas9 Knock-Out or Knock-In production, our CRISPR team needs the following:

Gene Target Information:
- Gene ID and Sequence
  - Target species (e.g., human, mouse, etc.).
  - Ensure access to the complete gene annotation, including exons, introns, and regulatory elements.
  - Accession number or full gene sequence.
- Knock-Out Details
  - Determine which exon or region to disrupt (e.g., start codon or functional domain).
  - Consider off-target analysis to avoid unintended modifications.
- Knock-In Details
  - Define the exact sequence to introduce (e.g., SNP, tag, or entire gene).
  - Include homology arms (~500–1,000 bp on either side of the edit).
CRISPR Design Information:
- Guide RNA (gRNA) Design
  - Select target sites with minimal off-target potential using design tools (e.g., CRISPRscan, Benchling, or CHOPCHOP).
  - Optimal gRNA features: GC content ~40–60%, minimal self-complementarity.
- Choose the Cas protein suitable for the application:
  - We recommend SpCas9
Cell or Organism Information
- Cell Line or Organism
  - Species and cell line specifics (e.g., HEK293T, C2C12, hMSCs).
  - Doubling time and transfection efficiency.
- Delivery Method
  - Choose the most efficient delivery method based on the cell type:
    - Lipid-based transfection (e.g., Lipofectamine)
    - Electroporation/nucleofection
    - Viral vectors (AAV, adenovirus, lentivirus, retrovirus).
Knock-In Specifics
- Donor Template for Knock-In
  - Single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA).
  - Includes homology arms flanking the intended edit.
- Repair Pathway
  - Homology-directed repair (HDR) or non-homologous end joining (NHEJ).
Validation Plan
- Genotyping Assays
  - PCR amplification and sequencing (e.g., Sanger, NGS).
  - Restriction enzyme digestion for indel detection.
- Phenotypic Validation
  - Confirm the functional outcome of the edit using assays (e.g., Western blot for protein knockout, reporter assays for KI).
- Off-Target Analysis
  - Assess potential off-target edits with in silico tools and/or experimental validation.

CRISPR-Cas9 Technology in Biotechnology Research and Development

CRISPR technology has a wide range of applications across various fields, such as precision medicine research, because of its versatility and precision in editing genes and DNA sequences.

Companies and organizations like Excision BioTherapeutics, Cure Rare Disease, and CRISPR Therapeutics are several companies who utilize CRISPR-Cas9 in their groundbreaking platforms.

Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on CRISPR based research.