Custom CRISPR / Cas9 services
Quick turnaround CRISPR vectors, cassette design, and gene knock-out and knock-in kits
CRISPR technology has a wide range of applications across various fields, such as precision medicine research, because of its versatility and precision in editing genes and DNA sequences. The Cas9 protein in the CRISPR system acts as a molecular scissor that makes a targeted double-strand DNA break at a specific location in the genome, and the resulting repair process can lead to gene knockout, gene correction, or gene insertion, depending on the desired outcome and the presence of a repair template.
Companies and organizations like Excision BioTherapeutics, Cure Rare Disease, and CRISPR Therapeutics are several companies who utilize CRISPR/Cas9 in their groundbreaking therapeutics. Producing CRISPR vectors, cassettes, and knock-in and knock-out kits require extensive experience.
Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at Science Direct and Genetic Engineering and Biotechnology News are always collecting and publishing the latest information on CRISPR based research. Let’s advance precision medicine together!
Our CRISPR vector collection
CRISPR Cas9 vectors are generic constructs used in genome editing system. Our CRISPR team have designed a series of these constructs to deliver the necessary components of the CRISPR system into cells for targeted gene editing. A typical CRISPR vector includes the following components: Cas9 gene, gRNA expression cassette, selectable marker genes, (optional) reporter genes, origin of replication, polyadenylation signal, and (optional) homology arms.
Interested in producing your own custom CRISPR Cas9 vector? Our CRISPR team is ready to start your production. If you are unsure which vector is right for your research application, our CRISPR team can discuss your project. Below is a list of the CRIPSR vectors we currently provide.
| VECTOR NAME | DESCRIPTION |
|---|---|
| pGPS-Cas-T2A-Puro | In Vivo Expression sgRNA and Cas9 |
| pGPS-Nickase-T2A-Puro | In Vivo Expression sgRNA and Nickase Cas9 |
| pGPS-T7-sgRNA | T7 promoter in Vitro sgRNA production |
| pGPS-T7-Cas9 | T7 promoter in Vitro Cas9 RNA production |
| pGPS-Cas | In vivo Cas9 expression |
sgRNA construct cloning service
Our cloning team is also able to assist in designing, generating, and cloning sgRNAs for CRISPR-Cas9 gene editing experiments. You can choose any above sgRNA expression vector or custom vector free of charge.
Donor vector construction design services
CRISPR cassettes are essential components in the CRISPR Cas9 genome editing system. They are used to deliver the necessary genetic elements, including the Cas nuclease (e.g., Cas9) and guide RNA (gRNA), into the target cells or organisms to perform precise gene editing. Our team can design and construct the best donor vector in homology directed repair. You can select a pre-designed or request a custom cassette for the tag or drug selection. Below is a list of the standard donor cassettes we currently provide.
| CASSETTE | VECTOR |
|---|---|
| Cassette 1 | CMV-GFP-T2A-Puro-PA |
| Cassette 2 | CMV-mCherry-T2A-Blasticidin-PA |
| Cassette 2 | Luciferase-CMV-GFP-T2A-Puro-PA |
CRISPR knock-out and knock-in kit services
CRISPR knock-out aims to disrupt the function of a gene, often by introducing mutations that render the gene non-functional, while CRISPR knock-in aims to insert a specific genetic sequence into the genome to achieve a desired genetic change or addition.
A predesigned gene knock-out or knock-in kit is a whole product solution for researchers to knock-out or knock-in a gene of interest. Our kits contain a plasmid that expresses a specific gRNA and Cas9 protein, a negative and a donor vector with Luciferase-CMV-GFP-T2A-Puro cassette that allow drug selection of positive clones.
Not sure which kit is right for your research? Our team has created the below table to outline the key differences.
| KNOCK-OUT KIT: | |
|---|---|
| Objective | The primary goals of CRISPR knock-out are to disrupt, deactivate, or "knock out" the function of a specific gene. This is usually achieved by introducing small insertions or deletions (indels) into the target gene's DNA sequence. These indels can introduce frame-shift mutations that often result in non-functional proteins or lead to premature stop codons. |
| Outcome | The gene's function is disrupted, and it may no longer produce a functional protein, effectively "turning off" the gene. |
| Applications | Knock-out genome editing is used to study gene function, model diseases, and explore the consequences of gene inactivation. It is also used as a therapeutic strategy for diseases caused by overactive or harmful genes. |
| KNOCK-IN KIT: | |
|---|---|
| Objective | The primary goal of knock-in kits are to insert, add, or "knock in" a specific genetic sequence (usually a new or modified DNA segment) into the genome at a precise location. This allows for the introduction of desired genetic changes, such as adding a functional gene or correcting a mutated gene. |
| Outcome | A specific genetic sequence is integrated into the target gene or genomic location, leading to the expression of a modified or additional gene product. |
| Applications | Knock-in genome editing is used for various purposes, including introducing reporter genes, tagging proteins for tracking or visualization, correcting disease-causing mutations, or adding specific sequences for therapeutic purposes. |