Lentivirus Packaging Services

Quick Turnaround Lentivirus Packaging for Research and Development

Cloning Constructs Produced
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Lentiviral Vectors Packaged
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Plasmid DNA Preparations
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Let's Start Your Lentivirus Packaging Production

Below is a list of the research grade viral vector services we currently provide. If you’re unsure which packaging service is right for your research, our team is more than happy to discuss your project. While we stand by our quick turnaround service to ensure your research stays on schedule, our lead times often change, so be sure to check the lead time for your preferred service before you begin production.

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Purification GradeProduction ScaleStandard TiterProduction Volume Options
Crude (for in vitro)Cell-culture grade, Small scale, Large scale~10E8 TU/ml250 μl; 500μl; 1ml
Ultracentrifiguration purified (for in vitro or in vivo)Cell-culture grade, Small scale, Large scale~10E9 TU/ml250 μl; 500μl; 1ml

Our viral vector team uses a validated assay to measure the viral titer in all lentivirus productions. Viral titer is determined through RT-qPCR, utilizing primers that target the long-inverted terminal repeat (LTR) within the viral genome. 

Lentivirus Packaging Production Additional Options

Lentivirus cell options Although VSV-G pseudotyped lentivirus is common in most lentivirus based research applications, our team can produce productions with pseudotype coronavirus spike protein lentivirus, bald lentivirus lacking viral envelope protein, and integrase-deficient lentivirus.

Cell lines Our lab performs lentivirus transduction using a HEK293T cell line (Human Embryonic Kidney 293 Transient) because of its high transfection efficiency. To learn more, read our article on lentivirus packaging cell line.

Generational lentiviral vector options Currently we offer lentivirus productions using either second or third generation lentiviral vectors. To learn more, read our article on lentivirus packaging generations. If your research application requires first generation, feel free to contact our team for a referral. 

Transfection protocol Viral payload, cell line, cell type, and lentivirus generation can greatly impact transfection efficiency. Because of this, we produce every lentivirus packaging to our client’s lentivirus transfection protocol specifications. If you do not have a lentivirus transfection protocol, we can determine an appropriate one based on your research needs. To learn more, read our article on lentivirus transfection protocols

Lentivirus Packaging Transfection Process Overview Lentivirus Packaging Protocol

Lentivirus Packaging Production Overview

Lentivirus Packaging Size Limit

According to viral research, the lentivirus packaging size limit is 8-10 kb. Our colleagues at ScienceDirect have published research articles stating a limit of 8 kb¹ ² while other researchers published in Nature have stated a limit of 10 kb³. There are many factors during the lentivirus packaging process that may affect the size limit of a plasmid DNA construct. Learn more about lentivirus packaging size limit.

Quality Control

As mentioned above, all lentivirus productions have their titer levels measured with a RT-qPCR (Quantitative Reverse Transcription Polymerase Chain Reaction) assay. In addition, we can perform the following quality control tests upon request:

  • Fluorescence microscopy
  • Flow cytometry
  • Western blot

Plasmid DNA Requirements

To begin production, we require only 1 μg of your plasmid DNA. We cover all plasmid prep expenses for your lentivirus production. Our plasmid preparations are always performed in house and result in endotoxin free and animal free plasmid DNA. To learn more, read about our plasmid preparation services.

pLenti-CMV-GFP-2A-Puro

Need to Design Your Own Lentiviral Vector?

Our cloning team can help design and produce the custom pLenti plasmid DNA construct for your research. To learn more, read about our molecular cloning services.

Lentivirus Based Precision Medicine Research

Lentivirus is an exciting retroviral vector for precision medicine cell and gene therapy research. Lentiviral vectors have been used in clinical trials and commercial vaccines for the treatment of rare diseases such as immunodeficiencies and neurodegenerative storage diseases. Their application for the research and treatment of more frequent genetic and acquired diseases, including β-thalassemia, Parkinson’s disease, and chimeric antigen receptor-based immunotherapy of cancer, are also being explored.

Pharmaceutical companies like J&J and Bristol Myers Squibb use lentivirus in the research and development of CAR-T based therapies. The Alliance for Regenerative Medicine is keeping record of current cell and gene therapy products including lentivirus based therapies available in different markets across the world.

Want to learn more about the latest in lentivirus based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News are always collecting and publishing the latest information on lentivirus based research.