Molecular Cloning Services
Quick Turnaround Molecular Cloning Services for Research Grade Plasmid DNA Constructs
Let's Get Started on Your Plasmid DNA Cloning Construct
Our cloning team specializes in creating, modifying, amplifying, and designing plasmid DNA that aligns to specific research objectives. To produce the research grade plasmid DNA researchers need, our team has assembled a comprehensive collection of shuttle vectors, promoters, open reading frames (ORFs), tags, reporters, and inducible systems. The tables below provide more details.
You can begin production in one of two ways: send us your plasmid map or submit your project details on our request a quote form.
Not sure where to start? No worries. Begin with our step-by-step process to determine the key components of your plasmid construct. If more refinement is needed, our cloning team can provide additional guidance to ensure your project correctly fits your research application.
Step 1: Choose a Backbone
Our cloning team offers eight different viral vector backbones, each of which presents a unique set of advantages and disadvantages. The chart below outlines the differences between our backbones so you can select the most appropriate one for your project.
| Characteristic | AAV | Adenovirus | Lentivirus |
|---|---|---|---|
| Genome: | 4.8 kb (ssDNA) | 36 kb (dsDNA) | 9 kb (ssRNA) |
| Packaging Capacity: | 4.7 kb | 7.5 kb | 9 kb |
| Infection: | Most dividing and non-dividing cells | Most dividing and non-dividing cells | Most dividing and non-dividing cells |
| Transduction Efficiency: | Moderate | High | Moderate |
| Integration: | Non-integrating | Non-integrating | Integrating |
| Expression: | Transient or stable | Transient | Stable |
| Immunogenicity: | Very low | High | Low |
| Biosafety: | BSL-1 | BSL-2 | BSL-2 |
Step 2: Choose a Promoter
Your plasmid DNA construct’s promoter determines where your gene’s transcription is initiated. Our cloning team has assembled this brief reference list of promoters and applications based on cell tissue. A complete list of our shuttle vectors and promoters is coming soon.
| Cell/Tissue Type | Recommended Promoter |
|---|---|
| Muscle: | MCK (1,359 bp), 3xMCK (728 bp) |
| Liver: | ALB (2,351 bp), TBG (460 bp) |
| Neurons: | CaMKIIa (1,291 bp), CK0.4 (217 bp), CK1.3 (1,143 bp), c-Fos (1,677 bp), MeCP2 (229 bp), NSE (1,321 bp), SST (1,230 bp), Syn (471 bp) |
| Astrocytes: | GFAP (2,040 bp), GFAP104 (845 bp) |
| Oligodendrocytes: | MBP (1,311 bp) |
| Retina: | Rpe65 (718 bp) |
| Pancreas: | PDX1 (854 bp) |
| Heart: | aMHC (372 bp), cTnT (702 bp) |
| Agnostic: | CAG (944 bp), CMV (508 bp), EF1a (1,190 bp), EFFS (253 bp), PGK (399 bp), UBC (1,137 bp) |
Step 3: Choose an Open Reading Frame (ORF)
Our team has assembled a collection of human ORFs that can be easily shuttled into the viral vector you choose. We also offer mutagenesis services so you can further customize your plasmid construct. You may also provide your own DNA sequence so our cloning team can subclone it into a viral vector.
A complete list of our ORFs is coming soon.
Optional Step 4: Add a Tag, Reporter, and/or Inducible System
BioInnovatise offers a wide selection of tags, reporters, and inducible systems to customize your viral vectors. An N- or C-terminus reporter can be directly fused to the transgene or separated by other elements (e.g., IRES or 2A peptide). When requesting a quote or starting production, let our team know which you prefer.
| Genetic Element | Available Options |
|---|---|
| Tags: | 6xHis (18 bp), FLAG (24 bp), HA (27 bp), Myc (30 bp) |
| Reporters: | eGFP (720 bp), mCherry (711 bp), mStrawberry (711 bp), RFP (711 bp), LacZ (3,051 bp), Luciferase (1,653 bp) |
| Inducible Reporters: | DIO-GFP (1,110 bp), DIO-mCherry (1,101 bp), DIO-RFP (1,101 bp), DIO-LacZ (3,296 bp) |
| Inducible Systems: | Cre (1,032 bp), DIO (386 bp), tTA (1,008 bp) |
Optional Step 5: Package Plasmid Construct into Lentiviral Vector Particles
Our viral vector team works hand-in-hand with our cloning team and can easily package your custom plasmid DNA construct into lentiviral particles. For more information on our lentivirus packaging services, visit our lentivirus services page.
Molecular Cloning in Precision Medicine
Molecular cloning is a versatile technique that allows researchers to create the specific plasmid DNA constructs they need to research and develop cell and gene therapies, study pharmacogenomics, observe disease biomarkers, and develop vaccines.
Precision medicine research and development progresses everyday, and with it, the need for high quality custom plasmid DNA constructs.
Want to learn more about the latest in molecular cloning? Our colleagues at ScienceDirect, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on molecular cloning.
13 Taft Court Suite 220
Rockville, MD 20850
(301) 838-8675
© 2024 BioInnovatise, All Rights Reserved