Molecular cloning services
Quick turnaround molecular cloning services for research grade plasmid DNA constructs
Molecular cloning is a versatile technique in molecular biology allowing researchers to create the specific plasmid DNA constructs they need to research and develop cell and gene therapies, study pharmacogenomics, observe disease biomarkers, and develop vaccines.
Precision medicine research and development progresses everyday, and with it, the need for high quality custom plasmid DNA constructs.
Want to learn more about the latest in molecular cloning? Our colleagues at Science Direct, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering and Biotechnology News are always collecting and publishing the latest information on molecular cloning. Let’s advance precision medicine together!
Let's get started on your plasmid DNA cloning construct
Our cloning experts have extensive experience in the creation, modification, amplification, and design of plasmid DNA that align to specific research objectives. In order to produce the research grade plasmid DNA researchers need, our team has assembled an extensive collection of shuttle vectors, promoters, open reading frames (ORFs), tags, reporters, and inducible systems. See below tables for details.
Our production team facilitates can start your production in two ways, by sending us your plasmid map or submitting your project details in our request a quote form.
Not sure where to start? No worries, our team has created the below step by step process to determine the key components of a plasmid construct. Our cloning experts are also able to provide advice on how to get started with your molecular cloning project to best fit your research application.
Step 1: Choosing a backbone
Each viral vector offers a unique set of advantages and disadvantages for developing a plasmid DNA construct. This below chart outlines our available viral vector backbones to choose from and select details on their differences.
| CHARACTERISTIC | AAV | ADENOVIRUS | LENTIVIRUS |
|---|---|---|---|
| Genome: | 4.8 kb (ssDNA) | 36 kb (dsDNA) | 9 kb (SSRNA) |
| Packaging capacity: | 4.7 kb | 7.5 kb | 9 kb |
| Infection: | Most dividing and non-dividing cells |
Most dividing and non-dividing cells |
Most dividing and non-dividing cells |
| Transduction efficiency: | Moderate | High | Moderate |
| Integration: | Non-integrating | Non-integrating | Integrating |
| Expression: | Transient or stable | Transient | Stable |
| Immunogenicity: | Very low | High | Low |
| Biosafety: | BSL-1 | BSL-2 | BSL-2 |
Step 2: Choosing a promoter
The promoter your plasmid DNA construct has will determine where the transcription of a gene is initiated. Our cloning experts have assembled this brief reference list of promoters and application based on cell tissue. A complete list of our shuttle vectors and promoters is coming soon!
| CELL/TISSUE TYPE | RECOMMENDED PROMOTER |
|---|---|
| Muscle | MCK (1,359 bp), 3xMCK (728 bp) |
| Liver | ALB (2,351 bp), TBG (460 bp) |
| Neurons | CaMKIIa (1,291 bp), CK0.4 (217 bp), CK1.3 (1,143 bp), c-Fos (1,677 bp), MeCP2 (229 bp), NSE (1,321 bp), SST (1,230 bp), Syn (471 bp) |
| Astrocytes | GFAP (2,040 bp), GFAP104 (845 bp) |
| Oligodendrocytes | MBP (1,311 bp) |
| Retina Rpe65 | Rpe65 (718 bp) |
| Pancreas PDX1 | PDX1 (854 bp) |
| Heart aMHC | (372 bp), cTnT (702 bp) |
| Ubiquitous | CAG (944 bp), CMV (508 bp), EF1a (1,190 bp), EFFS (253 bp), PGK (399 bp), UBC (1,137 bp) |
Step 3: Choosing an open reading frame (ORF)
Our team has assembled a collection of human ORFs that can be easily shuttled into the viral vector of your choice. We also offer mutagenesis services if you would like to customize your plasmid construct further. You may also provide your own DNA sequence so our cloning team can subclone into a viral vector.
A complete list of our ORFs is coming soon!
Optional Step 4: Adding a tag, reporter, and or inducible system
BioInnovatise offers a wide selection of tags, reporters, and inducible systems that can be used to customize your viral vectors of choice during the production of your plasmid DNA construct. An N- or C-terminus reporter can be directly fused to the transgene or separated by other elements (e.g., IRES or 2A peptide), let our team know whichever you prefer when requesting a quote or starting your production.
| Genetic Element | Available Options |
|---|---|
| Tags | 6xHis (18 bp), FLAG (24 bp), HA (27 bp), Myc (30 bp) |
| Reporters | eGFP (720 bp), mCherry (711 bp), mStrawberry (711 bp), RFP (711 bp), LacZ (3,051 bp), Luciferase (1,653 bp) |
| Inducible reporters | DIO-GFP (1,110 bp), DIO-mCherry (1,101 bp), DIO-RFP (1,101 bp), DIO-LacZ (3,296 bp) |
| Inducible systems | Cre (1,032 bp), DIO (386 bp), tTA (1,008 bp) |
Optional Step 5: Packaging plasmid construct into lentiviral vector particles
Our viral vector experts work hand in hand with our cloning team and can easily package your custom plasmid DNA construct into lentiviral particles. For more information on our lentivirus packaging services, visit our lentivirus services page.
