Custom mutagenesis services
Quick turnaround mutagenesis services for research grade plasmid DNA constructs
Mutagenesis involves intentionally introducing specific genetic mutations into a DNA molecule. These mutations can be single nucleotide changes (point mutations), insertions, deletions, or other alterations in the DNA sequence. Researchers in precision medicine mutate plasmid DNA to create targeted genetic changes to correct or modify genes associated with diseases or to study their effects on disease mechanisms.
Mutagenesis plays a significant role in the development of personalized therapies and the understanding of genetic diseases.
Want to learn more about the latest in mutagenesis? Our colleagues at Science Direct, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering and Biotechnology News are always collecting and publishing the latest information on genetic mutation research. Let’s advance precision medicine together!
Site-directed mutagenesis:
BioInnovatise’s site-directed mutagenesis service advantages:
- Mutations for small and large plasmids: Any point mutations, insertions and deletions can be made on up to 18 kb plasmid
- High accuracy: Our high-fidelity DNA polymerase ensures the accuracy of site-directed mutation and guarantee any unwanted mutations on your construct.
- Multiple mutation sites can be introduced in one mutagenesis reaction.
Site-directed mutagenesis sample submission requirements:
- The complete sequence of the template (the target)
- Mutation specifications including points, insertion and deletion requirements
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA, however we can grow your plasmid DNA if you provide a plasmid map
Random mutagenesis:
Our random mutagenesis service modifies proteins using error-prone PCR.
BioInnovatise’s random mutagenesis service advantages:
- Expression or other vectors: Randomized DNA can be cloned into your choice of expression vector or viral vector.
- DNA sequencing: Our team confirms the desired mutation by DNA sequencing.
- Mutation frequency: Selection of mutation frequencies depending on the application of the study.
- Viral titer: Titration of your randomly mutated plasmid library.
Random mutagenesis sample submission requirements:
- The complete sequence of the template (the target)
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA, however we can grow your plasmid DNA if you provide a plasmid map
Site-directed random mutagenesis:
Our site-direct random mutagenesis service also modifies proteins using error-prone PCR.
BioInnovatise’s site-directed random mutagenesis service advantages:
- Expression or other vectors: Site-directed randomized DNA can be cloned into your choice of expression vector or viral vector.
- DNA sequencing: Our team confirms the desired site-directed mutation by DNA sequencing.
- Mutation frequency: Selection of site-directed mutation frequencies depending on the application of the study.
- Viral titer: Titration of your site-directed randomly mutated plasmid library.
Site-direct random mutagenesis sample submission requirements:
- The complete sequence of the template (the target) and indicated sites for mutation
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA, however we can grow your plasmid DNA if you provide a plasmid map
Mutated plasmid construct deliverable:
- 2 – 4 µg of research grade mutant construct plasmid DNA, higher quantities are available upon request
- Electronic project report with sequence alignments and original sequencing data
Need more than 2 - 4 µg of research grade plasmid DNA?
Our plasmid experts offer plasmid DNA preparation services if you require a larger volume. We have a flexible scale, from Miniprep (10 μg), Midiprep (100 μg), Maxiprep (1-2 mg) up to a gram level. Our high quality research grade plasmids will help you achieve highly efficient cell transfection and achieve your research objectives. Learn more about our plasmid prep services on our plasmid DNA prep page.