Mutagenesis Services
Quick Turnaround PCR and Transposon Mutagenesis Services for Research Grade Plasmid DNA
Let's Begin Your Mutagenesis Production
Our team offers Polymerase Chain Reaction (PCR) and transposon mutagenesis services, on constructs, both linear and circular, up to 18 kilobase pairs. Our site directed mutagenesis services can include point or substitution, insertion, and deletion mutations. To help you choose the right service for your research, our team has prepared an overview on the differences.
Mutagenesis service lead times can change, so be sure to check our current lead times.
PCR Site Directed Mutagenesis (Point/Substitution, Insertion, Deletion), Random Mutagenesis, and Site Saturation Overview
PCR Mutagenesis Services
PCR based mutagenesis continues to be an extremely common molecular biology technique for mutating plasmid DNA sequences. Using primers to meet your mutation requirements, our team can match your mutation requirements on any plasmid DNA construct up to 18 kilobase pairs. Mutagenesis production requirements range from a single sequence update to complete library construction for a specific sequence(s). Read more about mutagenesis primer designs below.
Site Directed Mutagenesis (Point/Substitution, Insertion, Deletion)
- Any point mutations, insertions, and deletions can be made on up to 18 kb plasmid.
- Our high-fidelity DNA polymerase ensures the accuracy of site directed mutation and guarantees no unwanted mutations.
- Multiple mutation sites can be introduced in one mutagenesis reaction.
Random Mutagenesis
- Randomized DNA can be cloned into your choice of expression vector or lentiviral vector.
- Our team confirms the desired mutation by DNA sequencing.
- Any mutation frequency can be performed depending on the stability of your construct.
Site Directed Random Mutagenesis
- Site directed randomized DNA can be cloned into your choice of expression vector or lentiviral vector.
- Our team confirms the desired mutation by DNA sequencing.
- Any mutation frequency can be performed depending on the stability of your construct.
Site Saturation Mutagenesis
- DNA can be cloned into your choice of expression vector or lentiviral vector.
- Our team confirms the desired mutation by DNA sequencing.
- Any targeted mutation frequency can be performed depending on the stability of your construct.
Transposon Mutagenesis Services
Our team provides transposon mutagenesis services on genome sequences including insertional mutagenesis genes or regulatory regions and transposon-induced rearrangements. We offer 5 different transposase proteins to choose including: Mos1 transposase, PiggyBac transposase, sleeping beauty transposase, Tn5 transposase, and Tn7 transposase. Learn more about transposon mutagenesis.
Plasmid Library Construction Services
Our cloning team provides site saturation mutagenesis or site directed mutagenesis, for library construction requests on DNA sequences where more precision and large sequence volume is required. Specific mutations can be achieved using oligonucleotide-based, overlap extension PCR, or synthetic oligonucleotide techniques, which we all offer. Learn more about site saturation mutagenesis.
If you are interested in plasmid DNA library construction, just include the library construction parameters and requirements when requesting a quote.
Sample Submission Requirements
PCR Site Directed Mutagenesis (Point/Substitution, Insertion, Deletion)
- The complete sequence of the template (the target)
- Mutation specifications including points, insertion, and deletion requirements
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA
PCR Random Mutagenesis
- The complete sequence of the template (the target)
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA
PCR Site Directed Random Mutagenesis
- The complete sequence of the template (the target) and indicated sites for mutation
- Maps and antibiotic resistance of the template and destination vectors
- 3 µg of wildtype plasmid DNA
PCR Site Saturation Service
- The complete sequence of the template (the target) and indicated sites for mutation
- The method of site saturation mutagenesis: oligonucleotide-based, overlap extension PCR, or synthetic oligonucleotide
- 3 µg of wildtype plasmid DNA
Transposon Mutagenesis
- The complete sequence of the template (the target)
- Your selection of selection markers, promoters, and other regulatory elements
- Your selection of transposase protein
- 3 µg of wildtype plasmid DNA
Mutated Plasmid Construct Deliverable
- 2-4 µg of research grade mutant construct plasmid DNA
- Electronic project report with sequence alignments and original sequencing data
Additional Resources
Site Directed Mutagenesis Primer Design
Site Directed Mutagenesis Primer Design
In site directed mutagenesis, or site specific mutagenesis, primers play an important role in introducing specific mutations into a DNA sequence (insertion, deletion, point, or substitution). Primers are short, single-stranded DNA sequences that are designed to be complementary to the target region in the DNA template. They serve as starting points for DNA synthesis by DNA polymerase.
Primers are designed with the desired mutation incorporated into their sequences. For our site directed mutagenesis productions, we procure the specific primer that is right for your mutation. Read more about site directed mutagenesis primer design.
Site Directed Mutagenesis Protocol
Site Directed Mutagenesis Protocol
Site directed mutagenesis protocols differ due to a combination of factors, including the nature of the target gene or DNA sequence, the desired outcome of the experiment involving the plasmid DNA construct that is being mutated, and the primers used. We perform site directed mutagenesis productions using our standard proprietary site directed mutagenesis protocol.
Mutagenesis Kit or Mutagenesis Service?
Mutagenesis Kit or Mutagenesis Service?
Some labs order site-directed mutagenesis kits from vendors to perform in-house mutations, however these kits have drawbacks: they require up to a week’s worth of time, as well as materials and equipment that are not included. They also produce a low yield. Our mutagenesis services save you time, allow for greater lengths of DNA constructs to be mutated, and require only 3 µg of wildtype plasmid DNA to begin. Read more about why mutagenesis service is easier than a kit.
Need More Than 2-4 µg of Research Grade Plasmid DNA
Need More Than 2-4 µg of Research Grade Plasmid DNA
Our plasmid team offer plasmid prep services if you require a larger volume. We have a flexible scale from plasmid midiprep (100 μg), plasmid maxiprep (1-2 mg), and large scale volume (up to 1 gram). Our plasmid prep will help you achieve highly efficient cell transfection to achieve your research objectives. Learn more about our plasmid DNA prep.
Mutagenesis in Biotechnology Research and Development
Mutagenesis plays a significant role in the development of personalized therapies, the understanding of genetic diseases, and our continued understanding of the human genome.
Want to learn more about the latest in mutagenesis? Our colleagues at ScienceDirect, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering & Biotechnology News continuously collects and publishes the latest information on genetic mutation research.
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