Mutagenesis Services

Quick Turnaround Mutagenesis Services for Research Grade Plasmid DNA Constructs

Cloning Constructs Produced
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Lentiviral Vectors Packaged
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Plasmid DNA Preparations
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Let's Begin Your Mutagenesis Production

Our team offers site directed  random, site directed random, transposon, and site saturation mutagenesis services, on constructs up to 18 kb. Our site directed mutagenesis service can include point or substitution, insertion, and deletion mutations. To help you choose the right service for your research, our team has prepared an overview on the differences.

Mutagenesis service lead times change often, so be sure to check our current lead times. And rest assured that our quick turnaround services will keep your research on schedule.

Insertion mutagenesis, Deletion mutagenesis, Point mutagenesis, Random mutagenesis, Site Directed Mutagenesis, Site Directed Random Mutagenesis
Site Directed Mutagenesis Service (Point/Substitution, Insertion, Deletion)Random Mutagenesis ServiceSite Directed Random Mutagenesis ServiceTransposon ServiceSite Saturation Mutagenesis Service
Service Capabilities• Any point mutations, insertions, and deletions can be made on up to 18 kb plasmid.
• Our high-fidelity DNA polymerase ensures the accuracy of site directed mutation and guarantees no unwanted mutations.
• Multiple mutation sites can be introduced in one mutagenesis reaction.
• Randomized DNA can be cloned into your choice of expression vector or lentiviral vector.
• Our team confirms the desired mutation by DNA sequencing.
• Any mutation frequency can be performed depending on the stability of your construct.
• Site directed randomized DNA can be cloned into your choice of expression vector or lentiviral vector.
• Our team confirms the desired mutation by DNA sequencing.
• Any mutation frequency can be performed depending on the stability of your construct.
• DNA can be cloned into your choice of expression vector or lentiviral vector.
• Our team confirms the desired mutation by DNA sequencing.
• Any targeted mutation frequency can be performed depending on the stability of your construct.
• Your choice of selection marker(s), promoter(s), other regulatory element(s), and choice of transposase protein: Mos1, PiggyBac, Sleeping beauty, Tn5, or Tn7.
• DNA can be cloned into your choice of expression vector or lentiviral vector.
• Our team confirms the desired mutation by DNA sequencing.
• Any targeted mutation frequency can be performed depending on the stability of your construct.
Insertion mutagenesis, Deletion mutagenesis, Point mutagenesis, Random mutagenesis, Site Directed Mutagenesis, Site Directed Random Mutagenesis

Site Directed Mutagenesis (Point/Substitution, Insertion, Deletion), Random Mutagenesis, and Site Directed Random Mutagenesis Overview

Sample Submission Requirements

Site Directed Mutagenesis Service: (Point/Substitution, Insertion, Deletion)Random Mutagenesis ServiceSite Directed Random Mutagenesis ServiceTransposon ServiceSite Saturation Service
• The complete sequence of the template (the target)
• Mutation specifications including points, insertion, and deletion requirements
• Maps and antibiotic resistance of the template and destination vectors
• 3 µg of wildtype plasmid DNA
• The complete sequence of the template (the target)
• Maps and antibiotic resistance of the template and destination vectors
• 3 µg of wildtype plasmid DNA
• The complete sequence of the template (the target) and indicated sites for mutation
• Maps and antibiotic resistance of the template and destination vectors
• 3 µg of wildtype plasmid DNA
• The complete sequence of the template (the target)
• Your selection of selection markers, promoters, and other regulatory elements
• Your selection of transposase protein
• 3 µg of wildtype plasmid DNA
• The complete sequence of the template (the target) and indicated sites for mutation
• The method of site saturation mutagenesis: oligonucleotide-based, overlap extension PCR, or synthetic oligonucleotide
• 3 µg of wildtype plasmid DNA
Insertion mutagenesis, Deletion mutagenesis, Point mutagenesis, Random mutagenesis, Site Directed Mutagenesis, Site Directed Random Mutagenesis

Site Directed Insertion, Deletion or Point/Substitution Mutation, Random Mutation, and Site Directed Random Mutation Production

Mutated Plasmid Construct Deliverable

  • 2-4 µg of research grade mutant construct plasmid DNA
  • Electronic project report with sequence alignments and original sequencing data

Transposon Mutagenesis

Our team provides transposon mutagenesis services on genome sequences including insertional mutagenesis for insertion into genes or regulatory regions and transposon-induced rearrangements. We offer 5 different transposase proteins to choose including: Mos1 transposase, PiggyBac transposase, sleeping beauty transposase, Tn5 transposase, and Tn7 transposase. Learn more about transposon mutagenesis

Site Saturation Mutagenesis

Our cloning team provides site saturation mutagenesis services on DNA sequences where more precision is required than random mutagenesis. Specific mutations can be achieved using oligonucleotide-based, overlap extension PCR, or synthetic oligonucleotide techniques, which we all offer. Learn more about site saturation mutagenesis

Site Directed Mutagenesis Primer Design

In site directed mutagenesis, primers play an important role in introducing specific mutations into a DNA sequence (insertion, deletion, point, or substitution). Primers are short, single-stranded DNA sequences that are designed to be complementary to the target region in the DNA template. They serve as starting points for DNA synthesis by DNA polymerase.

Primers are designed with the desired mutation incorporated into their sequences. For our site directed mutagenesis productions, we procure the specific primer that is right for your mutation. Read more about site directed mutagenesis primer design.

Need More Than 2-4 µg of Research Grade Plasmid DNA?

Our plasmid team offer plasmid prep services if you require a larger volume. We have a flexible scale from plasmid midiprep (100 μg), plasmid maxiprep (1-2 mg), and large scale volume (up to 1 gram). Our plasmid prep will help you achieve highly efficient cell transfection to achieve your research objectives. Learn more about our plasmid DNA prep

Site Directed Mutagenesis Protocol

Site directed mutagenesis protocols differ due to a combination of factors, including the nature of the target gene or DNA sequence, the desired outcome of the experiment involving the plasmid DNA construct that is being mutated, and the primers used. We perform site directed mutagenesis productions using our standard proprietary site directed mutagenesis protocol.

Mutagenesis Kit or Mutagenesis Service?

Some labs order site-directed mutagenesis kits from vendors to perform in-house mutations, however these kits have drawbacks: they require up to a week’s worth of time, as well as materials and equipment that are not included. They also produce a low yield. Our mutagenesis services save you time, allow for greater lengths of DNA constructs to be mutated, and require only 3 µg of wildtype plasmid DNA to begin. Read more about why mutagenesis service is easier than a kit

Mutagenesis in Biotechnology Research and Development

Researchers in precision medicine mutate plasmid DNA to create targeted genetic changes to correct or modify genes associated with diseases or to study their effects on disease mechanisms.

Mutagenesis plays a significant role in the development of personalized therapies and the understanding of genetic diseases.

Want to learn more about the latest in mutagenesis? Our colleagues at ScienceDirect, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering & Biotechnology News continuously collects and publishes the latest information on genetic mutation research.

Mutagenesis, site-directed mutagenesis, random mutagenesis, site-directed random mutagenesis