Primary Mouse Embryonic Fibroblasts (PMEFs) are isolated from mouse embryos at every 12.5 days. Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production.
Feeder cells can retain ES cells in non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that play roles in maintaining the pluripotency of ES cells.
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