Contributions to Discovery

Conversion of Human Multidrug Transporter P-Glycoprotein (Abcb1) from Drug Efflux to Uptake Pump Evidence for a Switch Modulating the Direction of Substrate Transport

2024 | Plasmid construct | dx.doi.org/10.2139/ssrn.5035776 | SSRN

Rapid intra-host diversification and evolution of SARS-CoV-2 in advanced HIV infection

2024 | Plasmid construct | doi.org/10.1038/s41467-024-51539-8

Development of a p62 biodegrader for autophagy targeted degradation

2023 | Plasmid construct | doi.org/10.1101/2024.11.19.624312dx.doi.org/10.2139/ssrn.5035776 | BioRxiv

Photoinhibition of the hERG potassium channel PAS domain by ultraviolet light speeds channel closing

2024 | Plasmid construct | doi.org/10.1016/j.bpj.2024.05.024 | Biophysical Journal

LPA3 Receptor Phosphorylation Sites: Roles in Signaling and Internalization

2024 | Plasmid construct | doi.org/10.3390/ijms25105508 | International Journal of Molecular Sciences

Discovery of Transcription Factors Involved in the Maintenance of Resident Vascular Endothelial Stem Cell Properties

2024 | Plasmid construct | doi.org/10.1080/10985549.2023.2297997 | Molecular and Cellular Biology

Clinical and functional spectrum of RAC2-related immunodeficiency

2024 | Plasmid construct | doi.org/10.1182/blood.2023022098 | Blood

Disruption of lysosomal nutrient sensing scaffold hyperactivates mTORC1 contributing to pathogenesis of a fatal neurodegenerative disease

2024 | Plasmid Lamtor1 construct | doi.org/10.1016/j.jbc.2024.105641 | Journal of Biological Chemistry

Photo-crosslinking hERG channels causes a U.V.-driven, state-dependent disruption of kinetics and voltage dependence of activation

2024 | Plasmid ERG construct | doi.org/10.1101/2024.01.09.574834 | bioRxiv

A Novel STAT1 Mutation Associated with Disseminated Mycobacterial Disease

2012 | Plasmid STAT1 construct | doi.org/10.1007/s10875-012-9659-2 | Journal of Clinical Immunology

Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter

2014 | Plasmid construct | doi.org/10.1016/j.jbiotec.2014.08.033 | Journal of Biotechnology

Signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations and disseminated coccidioidomycosis and histoplasmosis

2013 | Plasmid STAT1 construct | doi.org/10.1016/j.jaci.2013.01.052 | Journal of Allergy and Clinical Immunology

Pharmacological Characterization of the Imipridone Anticancer Drug ONC201 Reveals a Negative Allosteric Mechanism of Action at the D2 Dopamine Receptor

2021 | Plasmid gRNA construct | doi.org/10.1124/molpharm.121.000336 | Molecular Pharmacology

Novel signal transducer and activator of transcription 1 mutation disrupts small ubiquitin-related modifier conjugation causing gain of function

2018 | Plasmid STAT1 construct | doi.org/10.1016/j.jaci.2017.07.027 | Journal of Allergy and Clinical Immunology

Progressive Multifocal Leukoencephalopathy in Primary Immune Deficiencies: Stat1 Gain of Function and Review of the Literature

2016 | Plasmid construct | Plasmid STAT1 construct | doi.org/10.1093/cid/civ1220 | Clinical Infectious Diseases

SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth

2017 | Plasmid construct | doi.org/10.1158/0008-5472.CAN-16-2393 | Molecular and Cellular Pathobiology

SIRT2-Mediated Deacetylation and Tetramerization of Pyruvate Kinase Directs Glycolysis and Tumor Growth

2016 | Plasmid construct | doi.org/10.1158/0008-5472.CAN-15-2498 | Molecular and Cellular Pathobiology

SIRT1 Deacetylates FOXA2 and Is Critical for Pdx1 Transcription and β-Cell Formation

2013 | Plasmid construct | doi.org/10.7150%2Fijbs.7529 | International Journal of Biological Sciences

Repair of UV photolesions in xeroderma pigmentosum group C cells induced by translational readthrough of premature termination codons.

2013 | Plasmid construct | doi.org/10.1073/pnas.1312088110 | Proceedings of the National Academy of Sciences of the United States of America

Improving Treatment of Mycobacterial Infections

2016 | Plasmid STAT1 construct | repub.eur.nl/pub/94589/ | Erasmus University Rotterdam Department of Internal Medicine

MicroRNA-30 inhibits antiapoptotic factor Mcl-1 in mouse and human hematopoietic cells after radiation exposure

2016 | Plasmid construct | doi.org/10.1007/s10495-016-1238-1 | Apoptosis

Direct interaction of eag domains and cyclic nucleotide–binding homology domains regulate deactivation gating in hERG channels

2013 | Plasmid construct | doi.org/10.1085/jgp.201310995 | Journal of General Physiology

Novel Stat1 Mutation Disrupts Small Ubiquitin-related Modifier (Sumo) Conjugation Causing Gain of Function

2017 | Plasmid construct | doi.org/10.1016/j.jaci.2017.07.027 | Journal of Allergy and Clinical Immunology

Effects of Single Nucleotide Polymorphisms in Human KCNMA1 on BK Current Properties

2019 | Plasmid construct | doi.org/10.3389/fnmol.2019.00285 | Frontiers in Molecular Neuroscience

The hERG potassium channel intrinsic ligand regulates N- and C-terminal interactions and channel closure

2019 | Plasmid construct | doi.org/10.1085/jgp.201812129 | Journal of General Physiology

Identification and targeting of novel CDK9 complexes in acute myeloid leukemia

2019 | Plasmid CDK9 construct | doi.org/10.1182/blood-2018-08-870089 | Blood

Unbiased Analysis of the Functions and Interactions of Human Inflammasome Mutations

2021 | Plasmid construct | University of Pittsburgh

Novel Protein Kinase C-Mediated Control of Orai1 Function in Invasive Melanoma

2015 | Plasmid construct | doi.org/10.1128/MCB.01500-14 | Molecular and Cellular Biology

Gp29 LysA of mycobacteriophage TM4 can hydrolyze peptidoglycan through an N-acetyl-muramoyl-L-alanine amidase activity

2022 | Plasmid construct | doi.org/10.1016/j.bbapap.2021.140745 | Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics

Lysine 68 acetylation directs MnSOD as a tetrameric detoxification complex versus a monomeric tumor promoter

2019 | Plasmid construct | doi.org/10.1038/s41467-019-10352-4 | Nature Communications

Lifespan Kras mutation levels in lung and liver of B6C3F1 mice

2018 | Plasmid construct | doi.org/10.1002/em.22198 | Environmental and Molecular Mutagenesis

Structural mapping of patient-associated KCNMA1 gene variants.

2023 | Plasmid construct | doi.org/10.1101/2023.07.27.550850 | bioRxiv

An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome

2014 | Plasmid construct | doi.org/10.1038/ng.3089 | Nature Genetics

Evaluation of IRX Genes and Conserved Noncoding Elements in a Region on 5p13.3 Linked to Families with Familial Idiopathic Scoliosis and Kyphosis

2016 | Plasmid construct | doi.org/10.1534/g3.116.029975 | G3 Genes|Genomes|Genetics

Distinct mutations at the same positions of STAT3 cause either loss or gain of function

2016 | Plasmid construct | doi.org/10.1042/BCJ20160572 | Journal of Allergy and Clinical Immunology

HERG eag/PAS (ether-á-go-go/Per-Arnt-Sim) Domain: Elucidating its Role in Regulating Channel Gating in Physiology and Disease.

2012 | Plasmid construct | University of Mary Washington Department of Biology

Effects of heterologous expression of human cyclic nucleotide phosphodiesterase 3A (hPDE3A) on redox regulation in yeast

2016 | Plasmid construct | doi.org/10.1042/BCJ20160572 | Biochemical Journal

Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase

2016 | Plasmid construct | doi.org/10.1093/nar/gkw656 | Nucleic Acids Research

High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19

2021 | Plasmid construct | doi.org/10.1371/journal.ppat.1009431 | PLOS Pathogens

Architecture of the Bacteriophage T4 Activator MotA/Promoter DNA Interaction during Sigma Appropriation*

2013 | Plasmid construct | doi.org/10.1074/jbc.M113.475434 | Journal of Biological Chemistry

The N-linker region of hERG1a upregulates hERG1b potassium channels

2022 | Plasmid construct | doi.org/10.1016/j.jbc.2022.102233 | Journal of Biological Chemistry

Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms

2022 | Plasmid construct | doi.org/10.1038/s41467-022-29381-7 | Nature Communications

Molecular Modeling Predicts Novel Antibody Escape Mutations in the Respiratory Syncytial Virus Fusion Glycoprotein

2022 | Plasmid construct | doi.org/10.1128/jvi.00353-22 | Journal of Virology

Evidence for a Stereoselective Mechanism for Bitopic Activity by Extended-Length Antagonists of the D 3 Dopamine Receptor

2020 | Plasmid construct | doi.org/10.1021/acschemneuro.0c00425 | American Chemical Society Chemical Neuroscience

Negative reciprocal regulation between Sirt1 and Per2 modulates the circadian clock and aging

2016 | Plasmid construct | doi.org/10.1038/srep28633 | Scientific Reports

A structural basis for how ligand binding site changes can allosterically regulate GPCR signaling and engender functional selectivity

2020 | Plasmid construct | doi.org/10.1126/scisignal.aaw5885 | Science Signaling

Delineation of G Protein-Coupled Receptor Kinase Phosphorylation Sites within the D1 Dopamine Receptor and Their Roles in Modulating β-Arrestin Binding and Activation

2023 | Plasmid construct | doi.org/10.3390/ijms24076599 | International Journal of Molecular Sciences

Identification and Characterization of ML321: A Novel and Highly Selective D2 Dopamine Receptor Antagonist with Efficacy in Animal Models That Predict Atypical Antipsychotic Activity

2022 | Plasmid construct | doi.org/10.1021/acsptsci.2c00202 | ACS Pharmacology & Translational Science

Ppt1-deficiency dysregulates lysosomal Ca++ homeostasis contributing to pathogenesis in a mouse model of CLN1 disease

2022 | Plasmid construct | doi.org/10.1002/jimd.12485 | Journal of Inherited Metabolic Disease

R-type voltage-gated Ca2+ channels mediate A-type K+ current regulation of synaptic input in hippocampal dendrites

2022 | Plasmid construct | doi.org/10.1016/j.celrep.2021.110264 | Cell Reports

Characterization of Phytohormones from Botanical Supplements for Steroid Hormone Receptor Activity

2021 | Plasmid construct | University of Arkansas

Aberrant Acetylation of Mitochondrial Proteins, due to Loss of SIRT3, Results in Tumor Permissive Phenotypes and Increases Breast Cancer Malignancy Risk

2017 | Plasmid construct | Northwestern University

Discovery, Optimization and Characterization of ML417: A Novel and Highly Selective D3 Dopamine Receptor Agonist

2020 | Plasmid construct | doi.org/10.1021/acs.jmedchem.0c00424 | Journal of Medicinal Chemistry

Second-site suppressor mutations reveal connection between the drug-binding pocket and nucleotide-binding domain 1 of human P-glycoprotein (ABCB1)

2023 | Plasmid construct | doi.org/10.1016/j.drup.2023.101009 | Drug Resistance Updates

A polybasic motif in alternatively spliced KChIP2 isoforms prevents Ca2+ regulation of Kv4 channels

2019 | Plasmid construct | doi.org/10.1074/jbc.RA118.006549 | Journal of Biological Chemistry

Characterization of a Mutant RNA Polymerase in Bacteriophage T4 Prereplicative Transcription

2013 | Plasmid construct | New York University Department of Basic Medical Science

Functional Characterization of MnSod and ACSS1 Acetylation in Metabolism and Carcinogenesis

2021 | Plasmid construct | Northwestern University

Mechanisms of Diurnal Regulation of the BK current in the Brain’s Central Clock

2016 | Plasmid construct | University of Maryland School of Medicine

Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model

2017 | Plasmid construct | doi.org/10.1038/ncomms14612 | Nature Communications

Intégration du VIH-1 : Contrôle et régulation de l’interaction fonctionnelle entre l’intégrase et la chromatine

2016 | Plasmid construct | Université de Bordeaux

miR-124-3p Regulates FGF2–EGFR Pathway to Overcome Pemetrexed Resistance in Lung Adenocarcinoma Cells by Targeting MGAT5

2023 | Pc9 | doi.org/10.2147/CMAR.S274192 | Cancer Management and Research