Retrovirus Packaging Services
Quick Turnaround Retroviral Packaging for Research and Development
Let's Start Your Retrovirus Packaging Production
Below is a list of the research grade viral vector services we currently provide. If you’re unsure which packaging service is right for your research, our team is more than happy to discuss your project. Our lead times often change, so be sure to check the lead time for your preferred service before you begin production.
| Purification Grade | Production Scale | Standard Titer | Production Volume Options |
|---|---|---|---|
| Crude (for in vitro) | Cell-culture grade, small scale, large scale | ~10E8 TU/ml | 250 μl; 500μl; 1ml |
| Ultracentrifugation purified (for in vitro or in vivo) | Cell-culture grade, small scale, large scale | ~10E9 TU/ml | 250 μl; 500μl; 1ml |
Our viral vector team uses a validated assay to measure the viral titer in all retrovirus productions. Viral titer is determined through RT-qPCR, utilizing primers that target the long-inverted terminal repeat (LTR) within the viral genome.
Retrovirus Packaging Production Additional Options
Cell lines Our lab performs retrovirus transduction and lentivirus transduction using a HEK293T cell line (Human Embryonic Kidney 293 Transient) because of its high transfection efficiency. To learn more, read our article on lentivirus packaging cell line.
Transfection protocol Viral payload, cell line, and transgene can greatly impact transfection efficiency. Because of this, we produce every retrovirus packaging to our client’s retrovirus transfection protocol specifications. If you do not have a retrovirus transfection protocol, we can determine an appropriate one based on your research needs.
Retrovirus and Lentivirus Packaging Production Overview
Quality Control
As mentioned above, all retrovirus productions have their titer levels measured with a RT-qPCR (Quantitative Reverse Transcription Polymerase Chain Reaction) assay. In addition, we can perform the following quality control tests upon request:
- Fluorescence microscopy
- Flow cytometry
- Western blot
Retrovirus vs Lentivirus Packaging
Both lentivirus and retrovirus are versatile and effective vectors to deliver a genetic payload to a host cell. However there are differences between retrovirus and lentivirus packaging for many reasons. These considerations need to be acknowledged in order deliver proper research results. To learn more, read about lentiviral vs retroviral vectors.
Plasmid DNA Requirements
To begin production, we require only 1 μg of your plasmid DNA. We cover all plasmid preparation expenses for your retrovirus production. Our plasmid preparations are always performed in house and result in endotoxin free and animal free plasmid DNA. To learn more, read about our plasmid preparation services.
Retroviral Based Precision Medicine Research
Retrovirus is an exciting viral vector for cell and gene therapy research. Retroviral and lentiviral vectors have been used in clinical trials and commercial vaccines for the treatment of rare diseases such as immunodeficiencies and neurodegenerative storage diseases. Their application for the research and treatment of more frequent genetic and acquired diseases, including β-thalassemia, Parkinson’s disease, and chimeric antigen receptor-based immunotherapy of cancer, are also being explored.
Pharmaceutical companies like J&J and Bristol Myers Squibb use lentivirus in the research and development of CAR-T based therapies. The Alliance for Regenerative Medicine is keeping record of current cell and gene therapy products including retroviral based therapies available in different markets across the world.
Want to learn more about the latest in retroviral based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News are always collecting and publishing the latest information on lentivirus based research.
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