Improved Vectors and Genome-Wide Libraries For CRISPR Screening Research Services
BioInnovatise CRISPR Team
Updated April 15, 2025
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Engineering the Improvements
Researchers at MIT redesigned lentiviral vectors by optimizing nuclear localization signals (NLS), implementing human codon optimization of key components, and strategically repositioning the sgRNA expression cassette. This resulted in the development of lentiCRISPRv2, achieving a ten-fold increase in viral titer compared to earlier versions.
The creation of a dual-vector system (lentiCas9-Blast and lentiGuide-Puro) with separate delivery of Cas9 and sgRNA components yielded an astounding hundred-fold improvement in viral titer. The sgRNA libraries themselves were enhanced through more sophisticated computational algorithms for guide selection, focusing on targeting constitutively expressed exons while minimizing off-target effects. The researchers also expanded coverage to include microRNA regions and approximately 1,000 previously untargeted genes, creating more comprehensive screening capabilities.
Research Applications
These improved CRISPR screening libraries have found applications across an extraordinary range of biological and biomedical research areas. In oncology research, they enable the identification of genes essential for tumor growth, metastasis, and drug resistance mechanisms, as demonstrated initially with vemurafenib resistance in melanoma.
Immunology researchers employ these libraries to uncover critical factors in immune cell development, activation, and regulation. Drug discovery programs utilize them to rapidly identify and validate therapeutic targets and resistance mechanisms.
Developmental biologists apply these tools to elucidate gene functions in cellular differentiation and morphogenesis. The flexibility of having both single-vector systems (optimal for in vivo and primary cell applications) and dual-vector systems (ideal for establishing stable cell lines) allows researchers to select the most appropriate approach for their specific experimental context, whether studying complex tissues, rare primary cells, or standard laboratory cell lines.
Our Viral Vector And CRISPR Screening Services
The implementation of advanced CRISPR screening libraries requires several specialized services that our viral vector and CRISPR teams have.
These include high-throughput oligonucleotide synthesis for library construction, next-generation sequencing for library verification and screening results analysis, QC assays, and viral packaging services for library delivery.
Our team works across this entire workflow, with experience in custom library design optimized for specific research applications.
Let’s get started! After determining which gRNA selection you want to target our team can provide high-fidelity oligonucleotide synthesis and high titer viral packaging in AAV, adenovirus, lentivirus, or retrovirus vectors.
Our CRISPR and viral vector teams are excited to bring your project to life.
If you are unsure which gRNA you want to target or the specific library you want to use, contact our team.
Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on CRISPR based research.
Trusted By Top Researchers Across Disciplines and Therapeutic Areas
Subscribe for Product Releases, New Resources, and Company Updates
13 Taft Court Suite 220
Rockville, MD 20850
(301) 838-8675
Follow Us on LinkedIn
© 2025 BioInnovatise, All Rights Reserved