CRISPR-Cas9 Services: CRISPR Vectors
Quick Turnaround CRISPR Vectors for Research and Development
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Let's Start Your CRISPR-Cas9 Vector Production
Our CRISPR Vector Collection
CRISPR-Cas9 vectors are genetic constructs used in genome editing. Our CRISPR team has designed a series of these constructs to deliver the necessary components of the CRISPR system into cells for targeted gene editing. A typical CRISPR vector includes the following components: Cas9 gene, gRNA expression cassette, selectable marker genes, (optional) reporter genes, origin of replication, polyadenylation signal, and (optional) homology arms.
Below is a list of the CRIPSR vectors we currently provide for both in vivo expression and in vitro RNA production. If you’re unsure which vector is right for your research, our CRISPR team is happy to discuss your project with you.
| Vector | Description |
|---|---|
| pGPS-Cas-T2A-Puro | In vivo expression sgRNA and Cas9 |
| pGPS-Nickase-T2A-Puro | In vivo expression sgRNA and Nickase Cas9 |
| pGPS-T7-sgRNA | T7 promoter in vitro sgRNA production |
| pGPS-T7-Cas9 | T7 promoter in vitro Cas9 RNA production |
| pGPS-Cas | In vivo Cas9 expression |
CRISPR Vectors: In Vivo Expression
With in vivo expression systems like pGPS-Cas-T2A-Puro and pGPS-Nickase-T2A-Puro vectors, these gene delivery tools have several advantages and key characteristics including
- Mechanism: The vector delivers DNA encoding both the sgRNA and Cas9 protein. The target cells then transcribe and translate these components directly.
- Expression duration: Generally provides longer-lasting Cas9 and sgRNA expression, which can be either an advantage (sustained editing) or disadvantage (increased off-target effects).
- Delivery methods: Typically uses plasmid transfection, viral vectors (such as lentivirus or AAV), or nanoparticles.
- Selection: Often includes antibiotic resistance markers to select for successfully transfected cells.
- Applications: Well-suited for stable cell line generation, long-term experiments, and situations where sustained editing is beneficial.
CRISPR Vectors: In Vitro RNA Production
With in vitro production systems like your pGPS-T7-sgRNA and pGPS-T7-Cas9 vectors, these gene delivery tools have several advantages and key characteristics including
- Mechanism: RNA for sgRNA and/or Cas9 is produced outside the cell (in vitro) using T7 polymerase, then delivered directly to cells.
- Expression control: Provides precise control over dosage and timing as you deliver a defined amount of RNA.
- Duration: Results in transient activity as the RNA eventually degrades, reducing potential off-target effects.
- Reduced immunogenicity: Particularly important for therapeutic applications, as DNA vectors can trigger immune responses.
- Applications: Ideal for embryo editing, primary cells sensitive to transfection, and therapeutic applications where temporary editing is preferred.
Design Your Own CRISPR-Cas9 Vector
If you’re interested in producing your own custom CRISPR-Cas9 vector, our CRISPR team is ready to start your production. Our team has put together a list of frequently asked questions and answers about CRISPR vectors if you have questions. To create your own CRISPR vector, our team needs the following information:
- Target sequence information
- sgRNA sequence
- Vector backbone selection
- Promoter information
- Cloning strategy
- Cell type application
- Additional payload information if applicable
CRISPR Vector Sequencing Reports for IND Filing
Integrated Vector Sequencing Reports are a critical component of any Investigational New Drug application submitted to the FDA for gene therapy products. This analysis provides essential verification of the vector’s genetic integrity, identity, and stability. By documenting the complete nucleotide sequence of a therapeutic vector, identifying any potential sequence variants, and confirming genetic stability across manufacturing processes, this report addresses crucial regulatory requirements while demonstrating a commitment to product quality.
Our CRISPR team can provide Integrated Vector Sequencing Reports for CRISPR vectors, just ask when starting a project.
CRISPR-Cas9 Technology in Biotechnology Research and Development
CRISPR technology has a wide range of applications across various fields, such as precision medicine research, because of its versatility and precision in editing genes and DNA sequences.
Companies and organizations like Editas Medicine, CorriXR Therapeutics, and CRISPR Therapeutics are several companies who utilize CRISPR-Cas9 in their groundbreaking platforms.
Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on CRISPR based research.
Trusted By Top Researchers Across Disciplines and Therapeutic Areas
Subscribe for Product Releases, New Resources, and Company Updates
13 Taft Court Suite 220
Rockville, MD 20850
(301) 838-8675
Follow Us on LinkedIn
© 2025 BioInnovatise, All Rights Reserved