Retrovirus Packaging Service
Quick Turnaround Retroviral Packaging for Research and Development
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Let's Start Your Retrovirus Packaging Production
Retrovirus is a versatile and effective viral vector used in many areas of research and development including cell and gene therapy, immunology, and oncology. Our team is able to package your plasmid DNA construct into retrovirus particles with a variety of production options.
Below is a list of the research grade retroviral vector services we currently provide. If you’re unsure which packaging service is right for your research, our team is more than happy to discuss your project. Our lead times often change, so be sure to check the lead time for your preferred service before you begin production.
| Purification Grade | Standard Titer | Production Volume Options |
|---|---|---|
| Crude (for in vitro) | ~10E8 TU/ml | 250 μl; 500 μl; 1 ml |
| Ultracentrifugation purified (for in vitro or in vivo) | ~10E9 TU/ml | 250 μl; 500 μl; 1 ml |
Our viral vector team uses a validated assay to measure the viral titer in all retrovirus productions. Viral titer is determined through RT-qPCR, utilizing primers that target the long-inverted terminal repeat (LTR) within the viral genome.
Retrovirus Packaging Production Additional Options
Cell lines Our lab performs retrovirus transduction and lentivirus transduction using a HEK293T cell line (Human Embryonic Kidney 293 Transient) because of its high transfection efficiency. To learn more, read our article on retrovirus packaging cell lines.
Transfection protocol Viral payload, cell line, and transgene can greatly impact transfection efficiency. Because of this, we produce every retrovirus packaging to our client’s retrovirus packaging protocol specifications. If you do not have a retrovirus transfection protocol, we can determine an appropriate one based on your research needs.
Retrovirus Packaging Production Process
Quality Control
As mentioned above, all retrovirus productions have their titer levels measured with a RT-qPCR (Quantitative Reverse Transcription Polymerase Chain Reaction) assay. In addition, we can perform the following quality control tests upon request:
- Fluorescence microscopy
- Flow cytometry
- Western blot
Retrovirus vs Lentivirus Packaging
Both lentivirus and retrovirus are versatile and effective vectors to deliver a genetic payload to a host cell. However there are differences between retrovirus and lentivirus packaging for many reasons. These considerations need to be acknowledged in order deliver proper research results. To learn more, read about lentiviral vs retroviral vectors.
Plasmid DNA Requirements
To begin production, we require only 1 μg of your plasmid DNA. We cover all plasmid preparation expenses for your retrovirus production. Our plasmid preparations are always performed in house and result in endotoxin free and animal free plasmid DNA. To learn more, read about our plasmid preparation services.
Why Choose Retrovirus As A Viral Vector
Retrovirus vectors have several unique advantages that make them ideal for certain applications, particularly in gene therapy and stem cell modification such as:
- Stable Integration: Like lentivirus, retrovirus vectors integrate their genetic material into the host cell genome, allowing for long-term, stable expression. This is crucial for therapies requiring permanent changes to the patient’s genome, especially in hematopoietic stem cells and gene-modified cell therapies.
- Efficient Integration into Dividing Cells: Retrovirus vectors are highly efficient in dividing cells, making them particularly suitable for hematologic disorders and stem cell engineering. Retrovirus is ideal for gene therapies that target stem cells, as these cells are typically in a dividing state, where retroviral integration is most efficient.
- Lower Immunogenicity: Retrovirus vectors generally have lower immunogenicity than adenovirus vectors, which tend to provoke strong immune responses.
- Lower Risk of Immune Response: Retrovirus vectors tend to provoke less of an immune response compared to adenovirus, allowing them to be used in therapies requiring multiple doses.
- Proven Success in Stem Cell Therapies: Retrovirus vectors have been successfully used in clinical trials for gene therapy of hematopoietic disorders, such as Strimvelis (ADA-SCID) and Zynteglo (beta-thalassemia).
Integrated Vector Sequencing Reports for IND Filing
Integrated Vector Sequencing Reports are a critical component of any Investigational New Drug application submitted to the FDA for gene therapy products. This analysis provides essential verification of the vector’s genetic integrity, identity, and stability. By documenting the complete nucleotide sequence of a therapeutic vector, identifying any potential sequence variants, and confirming genetic stability across manufacturing processes, this report addresses crucial regulatory requirements while demonstrating a commitment to product quality.
Our viral vector team can provide Integrated Vector Sequencing Reports for viral vectors, just ask when starting a project.
Retroviral Based Biopharmaceutical and Biotechnology Research & Development
Retrovirus is an exciting viral vector for cell and gene therapy research. Retroviral and lentiviral vectors have been used in clinical trials and commercial vaccines for the treatment of rare diseases such as immunodeficiencies and neurodegenerative storage diseases. Their application for the research and treatment of more frequent genetic and acquired diseases, including β-thalassemia, Parkinson’s disease, and chimeric antigen receptor-based immunotherapy of cancer, are also being explored.
Pharmaceutical companies like J&J and Bristol Myers Squibb use lentivirus in the research and development of CAR-T based therapies. The Alliance for Regenerative Medicine is keeping record of current cell and gene therapy products including retroviral based therapies available in different markets across the world.
Want to learn more about the latest in retroviral based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News are always collecting and publishing the latest information on retrovirus based research.
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