Mouse Embryonic Fibroblast Cells
Six Different Strains and Antibiotic Resistant Marker Options For Research and Development
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Our Collection
Mouse embryonic fibroblasts are foundational components in stem cell research and molecular biology research. They contribute to the understanding of cellular processes and disease mechanisms, and are tools for therapeutic development.
Our mouse embryonic fibroblasts are derived directly from embryonic tissue rather than being passaged or cultured for an extended period. The cell selection of fibroblast cells are isolated from mouse embryos every 12.5 days. Have a technical question about our current inventory? Contact our team.
| Current Selection |
|---|
| CD-1 mouse |
| CF-1 mouse |
| Neomycin-resistant mice |
| Hygromycin-resistant mice |
| Neomycin-resistant mice, mitomycin C inactivated |
| Puromycin-resistant mice, mitomycin C inactivated |
For more detailed information, download our Product Data Sheet for CF-1 Primary Mouse Embryonic Fibroblast
Applications for Primary Mouse Embryonic Fibroblasts
Our several types of MEFs available, including those from different mouse strains (CD-1, CF-1) and those with various antibiotic resistance markers (neomycin, hygromycin, puromycin), some of which have been mitomycin C inactivated, allow researchers to advance their virology and gene delivery research in a number of ways.
- Feeder layers for stem cell culture – MEFs provide essential growth factors and matrix proteins for embryonic stem cells and induced pluripotent stem cells.
- Co-culture systems – Supporting the growth of other specialized cell types that benefit from paracrine signaling.
- Cell signaling studies – Investigating fundamental cellular pathways and molecular mechanisms.
- Drug discovery and toxicity screening – Testing compound efficacy and cytotoxicity
- Gene function analysis – Studying the effects of gene knockouts, knockdowns, or overexpression
- Reprogramming experiments – Converting MEFs to induced pluripotent stem cells or other cell types
- Extracellular matrix production – Generating natural ECM components for 3D culture systems
- Senescence and aging research – MEFs undergo predictable senescence, making them useful for aging studies
- Virus production – Packaging retroviral or lentiviral vectors for gene delivery
- Metabolic studies – Analyzing basic cellular metabolism and bioenergetics
Learn more about the top applications for mouse embryonic fibroblasts.
Why Use MEFs In Research and Development?
Natural Microenvironment
- MEFs provide a complex, physiologically relevant microenvironment that’s difficult to replicate with synthetic systems
- They secrete a diverse array of growth factors and cytokines in balanced proportions
- The natural extracellular matrix produced by MEFs contains multiple proteins, proteoglycans, and signaling molecules
Established Track Record
- Decades of published research using MEFs creates a valuable comparative baseline
- Well-characterized system with predictable behavior and extensive troubleshooting resources
- Proven reliability for maintaining pluripotency in stem cells
Technical Advantages
- MEFs provide physical protection by metabolizing toxic compounds in the culture
- They buffer pH changes in the medium through metabolic activity
- The contact-dependent signaling between MEFs and cultured cells enhances survival
Practical Considerations
- Cost-effective compared to many specialized media or substrates
- Versatile across multiple applications rather than application-specific solutions
- Robust support for challenging cell types that don’t thrive in defined systems
Above are the images of MEFs observed under 10X (Left) and 20X (Right) magnification.
Specific Applications Where MEFs Remain Superior
- Early-stage development of novel stem cell lines
- Hard-to-culture primary cells that need extensive paracrine support
- Comparison studies with historical data where method consistency is crucial
- Virus production where cellular machinery and capacity are important
- 3D co-culture models attempting to recapitulate complex tissue interactions
Important Characteristics
- Primary cells – Directly isolated from embryonic tissue at 12.5 days, not immortalized
- Limited lifespan – Typically undergo senescence after 5-7 passages
- Strain-specific properties – Different mouse strains (CD-1, CF-1) may have subtle differences
- Antibiotic resistance – Engineered MEFs with neomycin, hygromycin, or puromycin resistance enable selective culture
- Mitomycin C inactivation – Growth-arrested but metabolically active cells for feeder layers
- Mesenchymal origin – Express typical fibroblast markers and morphology
- Secretory profile – Produce growth factors, cytokines, and extracellular matrix components
Mouse Embryonic Fibroblasts in Precision Medicine
Monolayers of mitotically inactivated feeder cells are essential for successful cultivation of mouse embryonic stem (ES) cells. ES cells are used in gene targeting and knock out mouse production.
Feeder cells can retain ES cells in non-differentiated morphology and pluripotent properties. The feeder cells secrete several important growth factors into medium that play roles in maintaining the pluripotency of ES cells.
Want to learn more about the latest in mouse embryonic fibroblasts? Our colleagues at ScienceDirect continuously collect and publish the latest information and research in the field.
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