CRISPR-Cas9 Services: CRSIPR Cassette Production and Donor Vector Construction Design Service
Quick Turnaround CRISPR Cassettes and Donor Vectors for Research and Development
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Let's Start Your CRISPR-Cas9 Cassette and Donor Vector Project
Our CRISPR Cassette and Donor Vector Library
CRISPR cassettes are essential components in the CRISPR-Cas9 genome editing system. They are used to deliver the necessary genetic elements, including the Cas nuclease (e.g., Cas9) and guide RNA (gRNA), into the target cells or organisms to perform precise gene editing. Below is a list of the cassettes and donor vectors we have predesigned.
| Cassette | Vector |
|---|---|
| Cassette 1 | CMV-GFP-T2A-Puro-PA |
| Cassette 2 | CMV-mCherry-T2A-Blasticidin-PA |
| Cassette 3 | Luciferase-CMV-GFP-T2A-Puro-PA |
Presdeigned CRISPR Cassette and Donor Vector Advantages
Our predesigned CRISPR cassettes offer several significant advantages for researchers interested in gene editing applications including:
- Time and resource efficiency: Our library allows researchers to skip weeks of design, cloning, and optimization work.
- Technical advantages: Our predesigned cassettes and donor vectors are chosen for optimal expression in target systems.
- Experimental benefits: Predesigned cassettes and vectors offer multiple options in one system.
- Accessibility: Researchers without extensive molecular biology expertise can perform CRISPR experiments.
Cassette Production and Donor Vector Construction Design Services
To begin your custom cassette and donor vector production that meets your specific research needs, we require the following information:
- Target Information:
- Target gene sequence
- Genomic location for integration
- PAM sequences available near your integration site
- Vector Design Components:
- Backbone vector selection such as AAV, lentivirus, baculovirus.
- Homology arms (left and right, typically 500-1000bp each)
- Selection markers
- Reporter genes (if applicable)
- Promoters appropriate for your expression needs
- gRNA Design:
- Target sequences for your CRISPR guides
- Appropriate promoter for guide expression
CRISPR Vector Sequencing Reports for IND Filing
Integrated Vector Sequencing Reports are a critical component of any Investigational New Drug application submitted to the FDA for gene therapy products. This analysis provides essential verification of the vector’s genetic integrity, identity, and stability. By documenting the complete nucleotide sequence of a therapeutic vector, identifying any potential sequence variants, and confirming genetic stability across manufacturing processes, this report addresses crucial regulatory requirements while demonstrating a commitment to product quality.
Our CRISPR team can provide Integrated Vector Sequencing Reports for CRISPR vectors, just ask when starting a project.
CRISPR-Cas9 Technology in Biotechnology Research and Development
CRISPR technology has a wide range of applications across various fields, such as precision medicine research, because of its versatility and precision in editing genes and DNA sequences.
Companies and organizations like Editas Medicine, CorriXR Therapeutics, and CRISPR Therapeutics are several companies who utilize CRISPR-Cas9 in their groundbreaking platforms.
Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on CRISPR based research.
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