Plasmid Library Construction Service
Quick Turnaround Plasmid DNA Library Construction Services
Molecular Cloning & Mutagenesis Projects Completed
Viral Vector Packaging Solutions Delivered
Plasmid DNA Preparations Successfully Produced
Let's Design Your Plasmid DNA Library
Our team offers plasmid DNA library construction and design services for both bacteria libraries and phage libraries.
Depending on your research application, our team can help design parameters and produce the library that makes sense for your research.
If you already know your plasmid DNA library requirements, you can reach out to our team to begin your production. If you are unsure about your requirements and design parameters, our team has assembled resources below and outlined our service capabilities. You can always contact our plasmid DNA team for additional guidance.
Plasmid DNA service lead times can change, so be sure to check our current lead times.
Bacteria Library and phLibrary Construction Services
The plasmid DNA team at BioInnovatise has consulted and produced many different plasmid libraries, both bacteria and phage, for discovery phase cell and gene therapy, oncology, and immunotherapy research. Our capabilities, sample submission requirements, and deliverables for each type of plasmid library do differ. However for both plasmid library construction services, we have a starting construct size limit of 3 kilobase pairs. If you have a construct larger than 3 kb or have a low fidelity/copy plasmid, please ask our team for a consultation.
Here is a quick overview on the key differences between bacteria libraries and phage libraries.
| Feature | Bacteria Library | phLibrary (Phage Library) |
|---|---|---|
| Biological carrier | Bacteria (e.g., E. coli) | Bacteriophage (phage particles) |
| Genetic material | Plasmids or vectors inside bacteria | DNA inserted in phage genome coding displayed peptides |
| Display | No display on surface; gene inside bacteria | Peptides/proteins displayed on phage coat |
| Screening type | DNA-based cloning and expression | Binding and interaction via displayed proteins |
| Library Clone Production Maximum | 100,000 10⁴ | 1,000,000 10⁵ |
Our team can produce a variety of plasmid DNA library types including:
- Random Mutagenesis Library (see our random mutagenesis services for non-library construction mutation projects)
- Site-directed Mutagenesis Library (see our site directed mutagenesis services for non-library construction mutation projects)
- Combination Library
- Genomic / cDNA Library
Sample Submission Requirements
Bacteria Library:
- The complete sequence of the vector, backbone type, plasmid map (if possible)
- Library parameters including strategy, target diversity, and coverage level. Our facility can produce up to 10⁴ variants for a bacteria library.
- 3 µg of wildtype plasmid DNA
phLibrary:
- The complete sequence of the vector, backbone type, plasmid map (if possible)
- Library parameters including strategy, target diversity, and coverage level. Our facility can produce up to 10⁵ variants for a phage library.
- 3 µg of wildtype plasmid DNA
Plasmid Construction Library Deliverable
- 2-4 µg of research grade of the plasmid library
- Electronic project report with sequence alignments, original sequencing data (if requested), and sample selection sequence verifications
Need More Than 2-4 µg of Research Grade Plasmid DNA
Our plasmid team offers plasmid prep services for amplified libraries. We have a flexible scale from plasmid midiprep (100 μg), plasmid maxiprep (1-2 mg), and large scale volume (up to 1 gram). Our plasmid prep will help you achieve highly efficient cell transfection to achieve your research objectives. Learn more about our plasmid DNA prep.
Plasmid DNA Integrated Vector Sequencing Reports for IND Filing
Integrated Vector Sequencing Reports are a critical component of any Investigational New Drug application submitted to the FDA for gene therapy products. This analysis provides essential verification of the vector’s genetic integrity, identity, and stability. By documenting the complete nucleotide sequence of a therapeutic vector, identifying any potential sequence variants, and confirming genetic stability across manufacturing processes, this report addresses crucial regulatory requirements while demonstrating a commitment to product quality.
Our plasmid DNA team can provide Integrated Vector Sequencing Reports for plasmid DNA vectors, just ask when starting a project.
Plasmid DNA in Biopharmaceutical and Biotechnology Research & Development
Precision medicine research and development progresses everyday, and with it, the need for high quality custom plasmid DNA constructs.
Want to learn more about the latest in plasmid DNA? Our colleagues at ScienceDirect, the American Society for Biochemistry and Molecular Biology, and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on plasmid DNA.
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