AAV Packaging Cell Line

Here are some commonly used cell lines for adenovirus production and transduction:

HEK293T Cells: Derived from human embryonic kidney cells transformed with adenovirus E1A gene, these cells are widely used for AAV packaging due to their high transfection efficiency, rapid growth, and ability to produce large quantities of viral vectors. The SV40 large T antigen present in HEK293T cells enables enhanced plasmid replication and protein expression, making them particularly advantageous for gene therapy and viral vector production. This is the preferred cell line for AAV packaging at BioInnovatise.

HEK293 (Human Embryonic Kidney 293) Cells: Human embryonic kidney cells created in 1973 by transforming primary kidney cells with sheared adenovirus type 5 DNA. These cells are prized in molecular biology for their robust growth, high transfection efficiency, and exceptional ability to produce recombinant proteins and viral vectors.

PerC6 Cells: Human retina-derived cell line engineered to express adenoviral E1 region genes. PerC6 cells are optimized for large-scale production and generate high viral yields with good safety profiles. This cell line is ideal for large scale manufacturing for cGMP productions.

Sf Cells: Insect cell line originally derived from fall armyworm ovarian tissue. Advantageous for baculovirus-mediated protein expression and viral vector production, particularly useful for large-scale manufacturing.

Sf9 Cells: Insect cell line derived from ovarian tissue of the fall armyworm Spodoptera frugiperda. Particularly advantageous for baculovirus expression systems and large-scale protein and viral vector production.

LSR Cells: Lung epithelial cells derived from a human lung adenocarcinoma. Provide an alternative to HEK293 cells with potentially different glycosylation profiles and cellular characteristics that can impact viral vector production and tropism.

A549 Cells: Human lung carcinoma cell line that is useful for viral production due to their epithelial-like characteristics and ability to support viral replication.