CRISPR Cas9 Services
Quick Turnaround CRISPR Vectors, Cassette Design, and Gene Knock Out and Knock In Services
Our CRISPR Vector Collection
CRISPR Cas9 vectors are genetic constructs used in genome editing. Our CRISPR team has designed a series of these constructs to deliver the necessary components of the CRISPR system into cells for targeted gene editing. A typical CRISPR vector includes the following components: Cas9 gene, gRNA expression cassette, selectable marker genes, (optional) reporter genes, origin of replication, polyadenylation signal, and (optional) homology arms.
If you’re interested in producing your own custom CRISPR Cas9 vector, our CRISPR team is ready to start your production. Our team has put together a list of frequently asked questions and answers about CRISPR vectors if you have questions.
Below is a list of the CRIPSR vectors we currently provide. If you’re unsure which vector is right for your research, our CRISPR team is happy to discuss your project with you.
| Vector | Description |
|---|---|
| pGPS-Cas-T2A-Puro | In vivo expression sgRNA and Cas9 |
| pGPS-Nickase-T2A-Puro | In vivo expression sgRNA and Nickase Cas9 |
| pGPS-T7-sgRNA | T7 promoter in vitro sgRNA production |
| pGPS-T7-Cas9 | T7 promoter in vitro Cas9 RNA production |
| pGPS-Cas | In vivo Cas9 expression |
sgRNA Construct Cloning Services
Our cloning team is also able to assist in designing, generating, and cloning sgRNAs for CRISPR Cas9 gene-editing experiments. You can choose any of the above sgRNA expression vectors or a custom vector free of charge.
Cassette Production and Donor Vector Construction Design Services
CRISPR cassettes are essential components in the CRISPR Cas9 genome editing system. They are used to deliver the necessary genetic elements, including the Cas nuclease (e.g., Cas9) and guide RNA (gRNA), into the target cells or organisms to perform precise gene editing. Our team can design and construct the best donor vector in homology-directed repair. You can select a pre-designed cassette or request a custom cassette for the tag or drug selection. Below is a list of the standard donor cassettes we currently provide. Learn more about CRISPR Cas9 cassettes.
| Cassette | Vector |
|---|---|
| Cassette 1 | CMV-GFP-T2A-Puro-PA |
| Cassette 2 | CMV-mCherry-T2A-Blasticidin-PA |
| Cassette 3 | Luciferase-CMV-GFP-T2A-Puro-PA |
CRISPR Knock Out and CRISPR Knock In Services
CRISPR knock out aims to disrupt the function of a gene, often by introducing mutations that render the gene non-functional, while CRISPR knock in aims to insert a specific genetic sequence into the genome to achieve a desired genetic change or addition.
Our CRISPR knock out or knock in services are a whole product solution for researchers to knock out or knock in a gene of interest. Our production deliverables contain a plasmid that expresses a specific gRNA and Cas9 protein, a negative, and a donor vector with Luciferase-CMV-GFP-T2A-Puro-PA cassette (unless otherwise noted) that allow drug selection of positive clones. Learn why a CRISPR Cas9 service is preferrable to a DIY kit.
Not sure which service is right for your research? The table below outlines the key differences.
| CRISPR Knock Out Service | |
|---|---|
| Objective | Knock out productions disrupt or deactivate (“knock out”) the function of a specific gene by introducing small insertions or deletions (indels) into a target gene’s DNA sequence. These indels can introduce frame-shift mutations that often result in non-functional proteins or lead to premature stop codons. |
| Outcome | The gene’s function is disrupted and it may no longer produce a functional protein, effectively “turning off” the gene. |
| Applications | Knock out genome editing is used to study gene function, model diseases, and explore the consequences of gene inactivation. It is also used as a therapeutic strategy for diseases caused by overactive or harmful genes. |
| CRISPR Knock In Service | |
|---|---|
| Objective | Knock in productions insert or add (“knock in”) a specific genetic sequence (usually a new or modified DNA segment) into a genome at a precise location, introducing a desired genetic change, such as adding a functional gene or correcting a mutated gene. |
| Outcome | A specific genetic sequence is integrated into the target gene or genomic location, leading to the expression of a modified or additional gene product. |
| Applications | Knock in genome editing is used for various purposes, including introducing reporter genes, tagging proteins for tracking or visualization, correcting disease-causing mutations, or adding specific sequences for therapeutic purposes. |
Sample Submission Requirements for Knock Out or Knock In Services
To get started on your CRISPR Cas9 Knock Out or Knock In production, our CRISPR team needs the following:
- 3 µg of wildtype plasmid DNA. If you need plasmid amplification or even higher volumes of plasmid DNA, let our team know. Learn about our quick turnaround plasmid prep services.
- The complete genome sequence you want to target.
- The desired sequence edits: insertions, deletions, or other modifications.
CRISPR Cas9 Technology in Biotechnology Research and Development
CRISPR technology has a wide range of applications across various fields, such as precision medicine research, because of its versatility and precision in editing genes and DNA sequences.
Companies and organizations like Excision BioTherapeutics, Cure Rare Disease, and CRISPR Therapeutics are several companies who utilize CRISPR Cas9 in their groundbreaking platforms.
Want to learn more about the latest in CRISPR genome-editing based research? Our colleagues at ScienceDirect and Genetic Engineering & Biotechnology News continuously collect and publish the latest information on CRISPR based research.
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